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KMID : 0613820070170010064
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Journal of Life Science
2007 Volume.17 No. 1 p.64 ~ p.69
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Effect of fisetin on UVB-induced apoptosis and DNA single strand breaks in NIH3T3 cells
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Jeong Se-Jin
Kim Don-Young
Han Seol-Hee
Shin Sang-Min
Cha Jae-young
Park Nou-Bog
Lee Jung-Sup
Park Jong-Kun
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Abstract
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In the present study, we have investigated the effect of fisetin on the apoptosis and DNA single strand breaks in ultraviolet light B (UVB)-exposed NIH3T3 cells. Exposure of cells to UVB light (200 J/㎡) and postincubation in growth medium for 48 hr resulted in about 50% of cells with apoptotic nuclear fragmentation. Addition of various concentrations of fisetin in the postincubation medium, however, significantly reduced the apoptotic nuclear fragmentation as compared with the values expected when the effects are additive and independent. DNA single strand breaks induced by UVB exposure were also significantly decreased by postincubation with fisetin. By Western blot analysis, fisetin postincubation was shown to attenuate the p53 upregulation upon UVB exposure. Furthermore, the decrease of proliferating cell nuclear antigen (PCNA) level upon UVB exposure was alleviated by fisetin postincubation. These results suggest that fisetin decrease the apoptosis and increae DNA repair in a possible association with alteration of p53 and PCNA levels in UVB-exposed cells.
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KEYWORD
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fisetin, UVB, DNA single strand breaks, apoptosis
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